zeiss axio vert a1 microscope  (Carl Zeiss)

Journal: Chembiochem

Article Title: Droplet Microfluidics‐Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization

doi: 10.1002/cbic.202500958

Figure Lengend Snippet: Fabrication and stability of biotin‐functionalized hybrid microgels loaded with MNPs. (A) Scheme illustrating droplet microfluidics‐assisted fabrication of biotin‐functionalized, poly(acrylamide)‐based hybrid microgels with MNPs. HµGel‐XB denotes biotinylated microgel, where X indicates biotin‐PEG‐acrylamide concentration in the precursor solution in % ( w/w ). Following ultrasonic treatment, the precursor solution is injected into microfluidic channels with flow‐focusing design (as shown in the AutoCAD design). Crosslinking of water‐in‐oil (W/O) emulsion droplets occurs via UV irradiation within the outflow tubing. (B) MNP stability in the precursor solution over time demonstrated by bright‐field microscopy images of purified microgels containing 1% ( w/w ) MNP and 2.5% biotin (HµGel‐2.5B) after 1.5 and 3.5 h of experimentation, respectively. Insets show uniform distribution of MNPs within the microgels and reveal consistent MNP loading during microfluidic processing over time. All scale bars denote 150 μm.

Article Snippet: Droplet formation was monitored through a high‐speed camera (Miro C110, Vision Research Inc., Wayne, NJ, USA) coupled to an inverted bright‐field microscope (10× objective lens, air, Axio Vert.A1, Carl Zeiss, Oberkochen, Germany).

Techniques: Concentration Assay, Injection, Emulsion, Irradiation, Microscopy, Purification

Journal: Chembiochem

Article Title: Droplet Microfluidics‐Assisted Fabrication of Magnetite Nanoparticle Hybrid Microgels for Facile Protein Immobilization

doi: 10.1002/cbic.202500958

Figure Lengend Snippet: Immobilization of sfGFP‐Mad10trunc‐His fusion protein on MNPs embedded inside pAAm‐based hybrid microgels. (A) 10× and (B) 40× bright‐field and corresponding CLSM microscopy images showing the selective binding of sfGFP‐Mad10trunc‐His to MNPs within HµGel‐1.25B after 3 days of incubation. All scale bars denote 150 µm.

Article Snippet: Droplet formation was monitored through a high‐speed camera (Miro C110, Vision Research Inc., Wayne, NJ, USA) coupled to an inverted bright‐field microscope (10× objective lens, air, Axio Vert.A1, Carl Zeiss, Oberkochen, Germany).

Techniques: Microscopy, Binding Assay, Incubation